Efficacy of Aqueous and Methanol extracts of Caesalpinia sappan L. and Mimosa pudica L. for their potential Antimicrobial activity

Aqueous and methanol extract of two medicinal plants of Caesalpinia sappan L. and Mimosa pudica L. were evaluated for their antimicrobial activities against Staphylococcus aureus NCIM 5021, Bacillus subtilis NCIM 2010, Escherichia coli NCIM 2118, Pseudomonous aeruginosa NCIM 5029, Klebsiella pneumoniae NCIM 2707, Proteus vulgaris NCIM 2027, Candida albicans NCIM 3102 and Aspergillus niger NCIM 545. The antibacterial activity of aqueous and methanol extracts was determined by agar disk diffusion and broth dilution method. The plant extracts were more active against Gram positive bacteria than against gram negative bacteria. The most susceptible bacteria were S.aureus, followed by B.subtilis, while more resistant bacteria were S.aureus, followed by E.coli. From the screening experiment, Caesalpinia sappan L. showed the best antibacterial activity; hence this plant can be further subjected to isolation of the therapeutic antimicrobials and further pharmacological evaluation.